Connecting live-cell imaging, cryofluorescence, and electron microscopy through microtechnology

John A. G. Briggs

MRC Laboratory of Molecular Biology, Cambridge, UK 

We are interested in the structure and the assembly mechanisms of enveloped viruses such as HIV and influenza virus, and of cellular trafficking vesicles including clathrin and COPI coated vesicles. To study these systems we are combining cryo-electron tomography with computational image processing and other complementary techniques. In combination, these methods can reveal the structures of assembled protein complexes “in situ” - directly within their functional, native environments.

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