Molecular strategies for regulating microtubule dynamics
Many aspects of eukaryotic cell function depend on the microtubule cytoskeleton. Microtubules are built from tubulin dimers and their intrinsic GTPase drives polymer dynamics. In vivo, an array of microtubule-associated proteins (MAPs) maintains tight control of microtubule stability and plasticity. However, the extent to which MAPs read and respond to the tubulin GTPase cycle and/or override it remains unclear. Our recent work has been aimed at elucidation of regulatory mechanisms of microtubule dynamics using cryo-electron microscopy and near-atomic resolution structure determination.